The Effect of Growth Regulators on in vitro Culture of Some Vitis vinifera L. Cultivars

Submitted: Apr 29, 2013; Accepted: May 30, 2013; Published: Jun 20, 2013 Abstract: This study was aimed to investigate the effects of growth regulators, including new one AN-16 (N-(3-phenylprop-2-yn-1-yl)-N-sodium butyldithiocarbamate, Patent 65377, Kazakhstan), on in vitro culture of some Vitis vinifera L. cultivars of Kazakhstan, European and Central Asia selections. Dormant buds of two cultivars: Saperavi and Riesling were placed in the IM medium, containing different concentrations of 6-benzylaminopurine (BAP) (0 μM; 4.4 μM; 8.8 μM) and -Naphthaleneacetic acid (NAA) (0 μM; 0.05 μM; 0.1 μM). For Saperavi, combination of hormones 4.4 μM BAP and 0.05 μM NAA in the IM medium gave more plants (198±2), compared to other combination of hormones, lowest number of plants were obtained with combination 8.8 μM of BAP and 0.1 μM NAA (51±1.7) (P <0.05). For Riesling, combinations of hormones 4.4 μM BAP/0.1 μM NAA and 8.8 μM BAP/0.1 μM NAA gave highest (133±1.6) and lowest (16±0.1) number of plants (P <0.05), respectively. Cabernet Franc, Rizamat and Bereke showed the maximum ability of the microshoot formation from apex. In vitro effect of 3indolelacetic acid (IAA) and AN-16 on rooting was tested for three grapevine varieties 'Saperavi', 'Merlot' and 'Red Glob'. Shoots (4-5 cm long) obtained by axillary buds proliferation were transferred from IM medium to rooting medium (1⁄2 MS) supplemented with 5.7 μmol plant hormone IAA or AN-16 (10 mg/l). The number of roots in rooting medium with AN-16 for all tested cultivars was significantly higher than with IAA (P <0.05).